Anti-IL-1 beta Antibody A00101-2 from BosterBio

Product Specs
ItemAnti-IL-1 beta Antibody
CompanyBosterBio
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA00101-2
ApplicationsELISA,IHC,WB
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Liquid (sterile filtered)
ReactivityHuman
HostRabbit
Brand/TrademarkBosterbio
NCBI Full Gene Nameinterleukin 1 beta
NCBI Gene AliasesIL-1, IL1-BETA, IL1F2, IL1beta
ClonalityPolyclonal
Quantity1mg
Concentration0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure
Research AreaAtherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation
StorageStore vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening
IsotypeIgG
ImmunogenThis antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli. The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1)
PurityThis is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW human IL-1ß and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1ß in the same formats using similar dilutions. The antiserum does not recognize human IL-1α. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured 31,000 precursor IL-1ß will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1ß exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1ß precursor in lysates
TargetIL1B